The workshop will be held on Monday, January 28th at the Palm Springs Convention Center in room Mesquite H from 12:30 – 2:00 pm. The Workshop title is Assay Optimization and Kinase Profiling in uHTS Format. Lunch will be served at this event.

Agenda

12:30PM-1:00PM - Sarah Shultz, MS, Applications Scientist, Promega
Advantages of Bioluminescence for uHTS Kinase Profiling

1:00PM-1:30PM - Jean Shieh, Applications Specialist, Labcyte
Moving to 1536-well plates with Echo®: Challenges and Solutions

1:30PM-2:00PM - Aoife Gallagher, PhD, Head of Applications, Deerac Fluidics
Low volume, high precision dispensing for the HTS environment

Successful kinase screening requires precise liquid handling, robust assays, reliable plates, and sensitive detection methods. With the increasing number of options available to the screener, it is a challenge to choose which are best suited for a particular application. This workshop demonstrates the complementarity of diverse technologies for a 1536-well kinase assay application.

Using a luminescent kinase technology platform from Promega, we demonstrate the optimization of kinase assays with the Deerac Fluidics Equator dispenser, using assay plates from Corning and with luminescent detection by BMG LABTECH’s Pherastar. Following assay optimization, the Labcyte Echo 555 liquid handler is used for ultra low-volume compound dispensing for screening and compound titration applications. Data show that miniaturization does not compromise quality when lower volume assays are required.

To register for the workshop please go to www.labautoworkshop.com